**** New Dual positive (NPTII & Sl. pFAST) PLUS anti-selection (FCY-UPP) iCas9 (pICSL22022)/Cas12a (pICSL22023) acceptor constructs added. Both constructs contain an AmilRFP negative selection cassette and accept guides in all positions (1-7) via Bpi1. ****
Parallel DNA assembly is the assembly of multiple fragments of DNA in a single reaction. Assembly techniques can be grouped into those that use restriction endonucleases, including BioBrick and Type IIS (e.g. Golden Gate), and those based on overlaps between adjacent parts such as Gibson Assembly, CPEC, SLIC, Ligase Cycling etc. There are several recent reviews that compare various parallel DNA assembly techniques. e.g. Patron (2014) and Casini et al (2015).