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Documentation

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  • SynBio@TSL News Items
  • DNA Assembly Techniques
  • Genome Engineering Technologies

Documents

SynBio@TSL News Items

  • 21st May 2022: Fascination of Plants Day
  • 3rd April 2017 - New TSL SynBio Website & Ordering System
  • 12th May 2016 - Note for users of Level2 Golden Gate acceptor plasmid pAGM4723
  • May 2022: Fascination of Plants

    DNA Assembly Techniques

  • DNA Assembly Techniques - An Outline
  • Type IIS mediated assembly

  • Download a quick guide to Type IIS cloning
  • Lab Protocols
  • Download a primer-design guide
  • Online Course
  • Golden Gate (Type II) Cloning: Hints, Tips and Trouble Shooting
  • Gibson Assembly

  • Gibson Assembly - How it works
  • Gibson Assembly - Protocol
  • USER Cloning

  • USER Cloning - How it works
  • Genome Engineering Technologies

  • Genome Engineering Technologies - An Outline
  • RNA-guided Cas9 (CRISPR)

  • RNA-guided Cas9-mediated genome engineering - Background
  • How to knock-out plant genes using RNA-guided Cas9
  • Transcription Activator-Like Effectors Nucleases (TALENS)

  • Transcription Activator-Like Effectors Nucleases (TALENS) - An Outline
  • Zinc Finger Nucleases

  • Zinc Finger Nucleases - An Outline